abm | CPS I and OTC Stable Expressing CHO (CHO-CPS45-22-OTC1-A19) Cell Line | T6178

abm | CPS I and OTC Stable Expressing CHO (CHO-CPS45-22-OTC1-A19) Cell Line | T6178

Carbamoyl phosphate synthetase I (CPS I) and ornithine transcarbamoylase (OTC), key enzymes in the urea cycle, were introduced to CHO cells by transfection with calcium phosphate to enable permanent CPS I and OTC gene expression. This cell line is suitable for use in the study of the urea cycle. Expression of these enzymes results in decreased ammonium ion production, making these cells ideal for research regarding the affect of ammonia levels on cell growth and productivity.

Biosafety:

II

Organism:

Hamster

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

CPS I, OTC, G418 (500 μg/mL), Hygromycin B (250 μg/mL)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm

Preservation:

1. Freeze Medium: Prigrow VIII with 40% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C