abm | CYP3A5 Double Deleted HuH-7 Cell Line | T6346

abm | CYP3A5 Double Deleted HuH-7 Cell Line | T6346

Cells were derived from HuH-7 cells. Cells were then genetically modified using CRISPR/Cas9 method with two guide RNAs to homozygously delete CYP3A5

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 72 hours

Seeding Density:

17,000 - 34,000 cells/cm^2. Split ratio of 1:2 to 1:5.

Markers:

Deletion of the 3B splice junction on both chromosomes. Very high CYP3A5 activity whereas the parental cell line has minimal activity.

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) PCR-based genotyping 2) Quantitatove real-time PCR 3)RNA Splicing Assay 4) Immunoblot Analysis 5) Midazolam and Tacrolimus Metabolism Assays

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C