abm | Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line | T3032

abm | Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line | T3032

The Notch-Delta signaling pathway plays a vital role in determining cell fates by mediating communication between neighboring cells during development. The Delta ligand has two important roles: it trans-activates Notch in neighboring cells, and cis-inhibits Notch in its own cell. Recent studies suggest that inhibitory same-cell interaction between the Notch receptor and its Delta ligand have significant consequences in the modes of intercellular signaling.

Biosafety:

II

Organism:

Chinese hamster

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line isPrigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1. qRT-PCR was used to assess the expression of Notch mRNA levels in the hN1G4^esn and hN1 cells and compared to early T-cell progenitors; 2. The Notch activity induced YFP signal were assess using IgG-Delta^ext fusion proteins fixed to plates at different concentrations and time-lapse recording.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C