abm | hPSEN1<sup>D257A<-sup> Stably Expressing MEF Psen1-2<sup>---<-sup> Cell Line | T6440

abm | hPSEN1^{D257A<-sup> Stably Expressing MEF Psen1-2---<-sup> Cell Line | T6440}

MEF Psen1/2^-/- cells were derived from E8.5 embryos which are neomycin and hygromycin-resistant, and were immortalized with SV40 large T antigen via transfection with the pMSSVLT plasmid. Human PSEN1 D257A mutant expression was established through retroviral transduction with pMSCV puro hPSEN1WT plasmid with site directed mutagenesis, and stable expression was determined via puromycin selection. PSEN1 (Presenilin 1) is a gene of interest in neuropathology, and diseases such as Alzheimer's disease (AD) in which patients with an inherited form of the disease carry mutations. abm offers a panel of Stable MEF expressing human PSEN1 wildtype and various human PSEN1 mutants for research.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Human PSEN1^D257A Neomycin (G418), hygromycin, and puromycin-resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C