abm | Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line | T3157

abm | Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line | T3157

Human T lymphoblasts are susceptible to HIV infection, and past studies had focused on the anti-HIV drug, 2‘,3‘-dideoxycytidine (ddC) for treatment. ddC relies on the nucleoside transport system and studies have linked the deoxycytidine kinase is involved in drug metabolism. The Human T Lymphoblast Stably Deoxycytidine Kinase Deficient Cell Line (AraC-8C) is a mutant clonal derivative of the CCRF-CEM cell line, which is also hypoxanthine-guanine phosphoribosyltransferase-deficient. AraC-8D cells are able to grow in the presence of 5 µM arabinosylcytosine and are deficient in nucleoside transport activities making it resistant to ddC unlike the parental cells. Additionally, the cells exhibit resistance to Ara-C, commonly used anti-cancer chemotherapy drug. It is valuable for research relating to the functions of nucleoside transport and related pathways, and for anti-cancer chemotherapeutic analysis.

Biosafety:

II

Organism:

Human

Source Organ:

Peripheral Blood

Growth Properties:

Suspension

Morphology:

Lymphoblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C