abm | KLK4-KLKP1 Stable Expressing RWPE-1 Cell Line | T6391

abm | KLK4-KLKP1 Stable Expressing RWPE-1 Cell Line | T6391

Pseudogenes have recently been implicated in gene regulation in cancer cells. The KLK4-KLKP1 pseudogene is found in more than 30% of prostate cancer patients and represents a rare case where a non-coding pseudogene is converted into a protein-coding gene. This stable cell line was generated by transducing the immortalized prostate cell line RWPE-1, using lentiviruses expressing the KLK4-KLKP1 gene. The transgene enhances cell proliferation, invasion, and tumour formation, as well as cause changes in gene expression. This cell line is useful for studying the role of KLK4-KLKP1 in prostate cancer development, and its possibility as a therapeutic target in drug screening studies.

Biosafety:

II

Organism:

Human

Source Organ:

Prostate

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

40,000 - 60,000 cells/cm²

Markers:

KLK4-KLKP1, blasticidin-resistant (3.5 μg/ml, Sigma-Aldrich)

Applications:

For Research Use Only

Doner Gender:

Male

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoat^TM T25 Flasks G299) or Applied Cell Extracellular Matrix G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C