abm | Nav2.1-β1-Contactin Stable Chinese Hamster Lung Cell Line | T3030

abm | Nav2.1-β1-Contactin Stable Chinese Hamster Lung Cell Line | T3030

Voltage-sensitive sodium channels are the membrane proteins responsible for initiation of the action potentials in most excitable cells. These ion channels are heteromultimeric structures that include the pore-forming α subunit in association with axillary β subunits. In the Na⁺² channel signaling complex, the glycosyl-phosphatidylinositol (GPI)-anchored protein, contactin (also known as F3, F11), has been shown to play an important role in the regulation of surface density of these channels. The Na_v2.1/ β1/Contactin Stable Chinese Hamster Lung (CHL) Cell Line stably expresses rat Na_v2.1 and β1 sodium channel subunits and mouse contactin. This cell line has been shown to express increased Na⁺² channel density in the surface membrane and serves as a useful model in studies relating to neuronal Na⁺² channel assembly and distributions.

Biosafety:

II

Organism:

Chinese hamster

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

rat Na_v2.1 and β1 sodium channel subunits and mouse contactin

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blotting and immunocytochemistry were used to detect the Na_v2.1, β1 and Contactin expression; 2) ³H-STX binding and current density measurements were used to assess the increased expression of Na⁺ channels.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C