abm | NF-κB Reporter CHO Cell Line (EL-1) | T6434

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SKU:
T6434
Availability:
5 to 7 Days Shipment
Size:
10⁶ cells/1.0 ml
Select Currency //4,173.00

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Description

abm | NF-κB Reporter CHO Cell Line (EL-1) | T6434

The NF-κB Reporter CHO Cell Line (EL-1) was constructed to express surface CD25 upon IL-1 or TNF stimulation by stable transfection of CHO fibroblast cells with the NF-κB-dependent reporter construct containing endothelial leukocyte adhesion molecule CD25, and the hygromycin vector pCEP4 (without CD14).

Biosafety:

II

Organism:

Chinese Hamster (C. griseus)

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-Like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm2

Markers:

Surface CD25 upon IL-1 or TNF stimulation Hygromycin B and Ciprofloxacin Resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

Flow cytometry using a FACScan microfluorometer & Western Blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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