abm | Stable CYP24A1 Expressing LS180 Cell Line | T6112

abm | Stable CYP24A1 Expressing LS180 Cell Line | T6112

Human Caucasian colon adenocarcinoma cell line LS 180 was transfected with reporter plasmid CYP24_minP-pNL2.1[Nluc/Hygro] to generate Stable CYP24A1 Expressing LS180 Cell Line. Stable CYP24A1 Expressing LS180 Cell Line contains much of the CYP24A1 promoter and therefore, can be used for study of transcriptional regulation of CYP24A1. This cell line allows for measurement of Vitamin D receptor transcriptional activity. This cell line can be used to profile VDR transcriptional activity in response to analogues of vitamin D, and intermediates in vitamin D2 and vitamin D3 metabolic pathways.

Biosafety:

II

Organism:

Human

Source Organ:

Digestive

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Luciferase

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Test of functionality after cryopreservation; 2) Test of long-term luciferase inducibility; 3) Assay performance

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C