abm | Stable HEK293T Expressing Human ECD GC-B (GCB-435) Cell Line | T6037

abm | Stable HEK293T Expressing Human ECD GC-B (GCB-435) Cell Line | T6037

The Stable HEK293T Expressing Human ECD GC-B (GCB-435) Cell Line contains the gene encoding for the extracellular ligand-binding domain (ECD) of the human GC-B. The expression of the ECD was done by transfecting HEK293T cells using a bicistronic mammalian expression vector containing the gene for the ECD. This stable cell line is not only convenient for the assessment of various drugs, it is also useful in characterizing other GCase receptors for which physiological functions are yet to be identified as well as for analyzing downstream signalling involving the ECD of the human GC-B.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 50,000 cells/cm²; Recommended split ratio is 1:2 to 1:12

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Via transfection of bicistronic mammalian expression vector, pIRES2–AcGFP1

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Western blot of a membrane preparation (60 lg of protein); 2) FACS-based selection based on fluorescence intensity from AcGFP1; 3) Selection with 0.2 µg/ml puromycin

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C