abm | Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line | T3195

abm | Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line | T3195

Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line is recommended for use to study the production of acetaldehyde and its effects in dysfunctions in alcoholic liver disease. The cell line expresses mouse ADH1 (Class 1 Alcohol Dehydrogenase) which oxidizes allyl alcohol to produce toxic acetaldehyde. The Va-13 cells may use used to investigate ethanol metabolism.

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfection with pIVL-2 vector encoding for mouse ADH1 (Class 1 Alcohol Dehydrogenase)

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line isPrigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) 400 μg/mL zeocin for selection; 2) Redox state was measured by levels of lactate and pyruvate released in culture medium.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C