abm | Stable PML Knockout Hep-2 Cell Line | T6038

abm | Stable PML Knockout Hep-2 Cell Line | T6038

The Stable PML Knockout Hep-2 Cell Line was generated from HEp-2 cells by transfection of CRISPR/Cas9 targeting exon 1 of promyelocytic leukemia protein (PML). PML is a component of nuclear domain 10 (ND10) and an antiviral effector of IFN- β. In the absence of PML, the antiviral effects of IFN- β are significantly reduced. This makes the Stable PML Knockout Hep-2 Cell Line a useful model in antiviral treatments and experiments. The PML gene is also associated with a wide range of important cellular processes including tumor suppression, transcriptional regulation, apoptosis, senescence, and DNA damage response which can all be further studied using this cell line.

Biosafety:

II

Organism:

Human

Source Organ:

Larynx

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

30,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Transfection of CRISPR/Cas9 targeting exon 1 of promyelocytic leukemia protein (PML)

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

Immunoblot analysis using anti-PML

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C