abm | Mouse Rad9 -/- Embryonic Stem Cells | T2510

Rad9, a checkpoint gene, plays a key role in the regulation of cellular response to DNA damage and the maintenance of genomic integrity. Mouse Rad9 -/- Embryonic Stem Cells were developed using a Cre/Loxp conditional knockout system. This cell line is suitable for research regarding the functions of Rad9, including the effect of loss of function in tumorigenesis.

Vector:

N/A

Species:

Mouse (M. musculus)

Organ:

Embryo

Passages:

Propagation:

Use of Gelatin Coating Solution (0.1%) is required for cell adhesion to the culture vessels. Grow cells in gelatin-coated culture vessels with the following conditions.
The base medium for this cell line is Knockout DMEM medium (Gibco).To make the complete growth medium, add fetal bovine serum to a final concentration of 15%, penicillin-streptomycin solution to a final concentration of 5%, 2mM L-glutamine, 10^(-4)M 2-Mercaptoethanol, 10^3 units/mL of ESGRO (Millipore), and 0.1mM non-essential amino acids solution (Gibco).
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Concentration:

10⁶ cells/vial

Induction Method:

N/A

Pluripotency:

N/A

Growth Properties:

Adherent

Morphology:

Polygonal

Markers:

Mrad9 -/- ES cells were transfected with pZeoSV2-Mrad9 and pZeoSV2-HRAD9 and then challenged with zeocin

Subculturing:

Cells should be kept at a density such that they can be split every second day at 1:6 dilution. Change medium between splits.

QC:

1) Southern blot; 2) PCR

Safety:

BioSafety Level II

Shipping:

Dry Ice

Storage:

-180°C