abm | REF-iPSC | T2020

N/A

Vector:

N/A

Species:

Rat

Organ:

Embryo

Passages:

N/A

Propagation:

The optimized medium for this cell line is Rat iPSC Culture Medium available from ABM (TM021). This cell line needs to be cultured on inactivated REF feeder cells (T2017). Atmosphere:air: 95%, CO₂: 5%; Temperature: 37.0°C.

Concentration:

10⁶ cells/vial

Induction Method:

N/A

Pluripotency:

N/A

Growth Properties:

N/A

Morphology:

N/A

Markers:

N/A

Subculturing:

1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new feeder cell culture vessel. 6. Incubate cultures at 37°C. Be sure to use inactivated REF feeder cells (T2017).

QC:

N/A

Safety:

BioSafety Level II

Shipping:

Dry Ice

Storage:

-180°C