MOUSE ANTI-DENGUE VIRUS SEROTYPE 4 MEMBRANE PROTEIN (NE5)

Mouse Anti Dengue Virus Serotype 4 (DENV4) Membrane Protein (NE5) is specific for the M protein of Dengue virus serotype 4, and does not cross-react with M protein from other Dengue virus serotypes.

PRODUCT DETAILS – MOUSE ANTI-DENGUE VIRUS SEROTYPE 4 MEMBRANE PROTEIN (NE5)

• Mouse anti-Dengue virus serotype 4 Membrane protein monoclonal IgG1 kappa antibody (clone NE5).
• Greater than 95% purity and buffered in PBS, pH7.4.

 

BACKGROUND

Dengue virus is a member of the flavivirus family, which includes Zika virus, West Nile virus and Japanese Encephalitis virus. Many of the proteins expressed by these viruses are very similar, and serological testing for these viruses can be complicated by problems of cross-reactivity. In this context, the use of highly purified antigens, that are glycosylated and folded as native proteins can be highly important in the development of accurate immunoassays.

The Envelope protein is a major structural protein expressed by the Dengue Virus. Envelope protein is a glycosylated protein of approximately 45-50kD, and which forms part of the virus capsid along with membrane protein (M). M protein is a small proteolytic fragment of the precursor prM protein and is produced during maturation of the viral particles in the secretory pathway. Dengue virus surface is composed of 180 copies the E and (pr/M) M proteins. Envelope protein is an important target for the human immune response, and many neutralising antibodies have been shown to recognise the Envelope protein.

With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics (WHO for Guidelines for Diagnosis, Treatment, Prevention & Control, 2009) As many as 100 million people are infected yearly. Dengue is caused by any one of four related viruses transmitted by mosquitoes. There are no vaccines available to prevent infection with dengue virus.

Early diagnosis of dengue and dengue serotypes is a vital step in patient management. However, many of the proteins expressed by Flaviviruses are very similar and serological testing is complicated by problems of cross-reactivity. In this context, the use of highly purified antigens, that are glycosylated and natively folded are crucial in the development of accurate and reliable immunoassays.

 

REFERENCES

• Dengue: guidelines for diagnosis, treatment, prevention and control. WHO, 2009.