PORCINE EPIDEMIC DIARRHEA VIRUS NUCLEOPROTEIN (INSECT CELLS), HIS-TAG

Recombinant Porcine Epidemic Diarrhea Virus (PEDV) antigen is a recombinant nucleocapsid or N protein derived from PEDV and manufactured using the baculovirus expression system.

PRODUCT DETAILS – PORCINE EPIDEMIC DIARRHEA VIRUS NUCLEOPROTEIN (INSECT CELLS), HIS-TAG

• C-terminal 6xHis tagged PEDV nucleoprotein (447 aa) (Accession No. KJ645707.1).
• Recombinant protein purified from baculovirus-infected insect cells.
• Purified protein was dialyzed against 10 mM Tris, pH 8.0, 0.1% Triton X-100 to remove the urea.
• Recombinant PEDV-NP-His expression confirmed in infected cells by Western with α-His and α-PEDV-NP specific antibodies. Tested in ELISA with α-PEDV-NP specific antibodies.

BACKGROUND

Porcine epidemic diarrhea virus (PEDV), an enveloped, single-stranded and positive-sense RNA virus, is the causative agent of porcine epidemic diarrhea (PED). PEDV belongs to the genus of Alphacoronavirus, the family of Coronaviridae and the subfamily of Coronavirinae. It was originally reported in Belgium and the United Kingdom and infection causes 80 to 100% fatality rate in suckling piglets (Jung et al., 2020).

PEDV has an approximately 28 kb long genome and consists of seven open reading frames (ORF), encoding non-structural or structural proteins. PEDV encodes several structural proteins, including the spike (S), envelope (E), membrane (M), and nucleoprotein (N). The N protein, which is the most abundant protein in the virus particle, provides the structural basis for the helical nucleocapsid surrounding the virus genome. The nucleoprotein also assists the correct folding, packaging and encapsidation of viral RNA. It can also regulate the host cell cycle to benefit virus infection. During the infection, PEDV nucleoprotein protein (PEDV-N) is mainly localized in the cytoplasm, but can also be found to a lesser extent in the nucleus. PEDV-N also antagonizes interferon-β production by sequestering the interaction between IRF3 and TBK1 (Huan et al., 2016)

REFERENCES

• Jung K, Saif LJ, Wang Q. Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and prevention and control. Virus Res. 2020 Sep;286:198045.
• Huan CC, Wang HX, Sheng XX, Wang R, Wang X, Liao Y, Liu QF, Tong GZ, Ding C, Fan HJ, Wu JQ, Mao X. Porcine epidemic diarrhea virus nucleoprotein contributes to HMGB1 transcription and release by interacting with C/EBP-β. Oncotarget. 2016 Nov 15;7(46):75064-75080