RABBIT IgG ANTI HCOV-HKU1 SPIKE (S1) POLYCLONAL ANTIBODY

Polyclonal antibody against human coronavirus HKU1 (HCoV-HKU1) spike subunit 1 (S1) protein. It shows some cross-reactivity towards other HCoV spike subunit 1 (S1) proteins and SARS-CoV S1 in ELISA, but not to SARS-CoV-2 S1 protein.

PRODUCT DETAILS – RABBIT IgG ANTI HCOV-HKU1 SPIKE (S1) POLYCLONAL ANTIBODY

• HCoV-HKU1 polyclonal antibody binds the S1 domain of the human coronavirus HKU1 spike subunit 1.
• This antibody was made by immunising rabbits with a recombinant HCoV-HKU1 spike subunit 1 antigen (REC31897).
• Protein A affinity purified from culture supernatant.
• Suitable for use in ELISA and WB.

BACKGROUND

The coronaviruses are a family of related RNA viruses within the order Nidovirales. They contain a positive-sense, single-stranded, 26-32kb RNA genome protected by a nucleocapsid of helical symmetry. Their viral capsids are surrounded by a lipid envelope, which is interrupted by trimeric Spike proteins that project from the capsid surface. Prior to early 2000, only two human coronaviruses were recognized: Human coronavirus 229E (HCoV-229E) and Human coronavirus OC43 (HCoV-OC43). However, in late 2002, a third human coronavirus (SARS-CoV) was implicated as the aetiological agent of severe acute respiratory syndrome (SARS) and since then, several more human coronaviruses have been identified, including HCoV-NL63 associated with upper and lower respiratory tract infections (Van der Hoek, 2004) and HCoV-HKU1 in patients with pneumonia (Woo et al., 2005), as well as the more lethal, Middle East respiratory syndrome (MERS) coronavirus and Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2). Like the other mild cold-causing human coronaviruses, HCoV-NL63, HCoV-OC43 and HCoV-HKU1, HCoV-229E has a worldwide distribution, with seasonal surges occurring in the winter months (Wat et al., 2004). Like the other cold-causing coronaviruses, reinfection with HCoV-229E is common. The reason for this is not yet clearly defined but may be due to a weak antibody response (Raoult et al., 2020).

REFERENCES

• Raoult, D. et al. (2020). Coronavirus infections: Epidemiological, clinical and immunological features and hypotheses. Cell Stress.
• Wat, D. et al. (2004). The Common Cold: A Review of the Literature. European Journal of Internal Medicine. 15(2): 79-88.
• Woo, PCY. et al. (2005). Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol79, 884–895.