RABBIT IgG ANTI-SARS-COV-2 SPIKE (S1) POLYCLONAL ANTIBODY

SARS-CoV-2 Spike (S1) polyclonal antibody is an antibody that recognizes the SARS-CoV-2 Spike glycoprotein, subunit 1 (S1) and is suitable for use in ELISA and WB. Antibody does not cross-react in ELISA with HCoV-229E full-length spike protein (REC31880).

PRODUCT DETAILS – RABBIT IgG ANTI-SARS-COV-2 SPIKE (S1) POLYCLONAL ANTIBODY

• SARS-CoV-2 Spike (S1) polyclonal antibody binds the S1 domain of the SARS-CoV-2 Wuhan-Hu-1 Spike protein, including the RBD and NTD. Antibody shows low reactivity to other SARS-CoV-2 variant RBD proteins (UK, REC31946; South African, REC31945; Indian, REC31971; Brazilian, REC31961).
• This antibody was made by immunising rabbits with a recombinant SARS-CoV-2 Spike subunit 1 antigen (REC31828).
• Protein A affinity purified from culture supernatant.
• Suitable for use in ELISA, IF and WB.

BACKGROUND

SARS-CoV-2 is part of the Coronaviridae family, subfamily Orthocoronavirinaes and includes the genera Alphacoronvirus, Betacoronavirus, Gammacoronavirus, and Deltacoronavirus. Alphacoronaviruses and betacoronaviruses typically infect mammals, whereas gammacoronoviruses and deltacoronaviruses generally infect avian species (and sometimes mammals). Two types of alphacoronaviruses (229E and NL63) and two types of betacoronaviruses (OC43 and HKU1) are pathogens of humans and cause the common cold. Whereas, SARS-CoV-1, the Middle Eastern respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2 (all betacoronaviruses) cause more severe disease in humans including, respiratory, gastrointestinal, hepatic, and neurologic diseases (Amanat & Krammer, 2020).

SARS-CoV-2 has a large single-stranded positive-sense RNA genome encoding for several open reading frames. One frame encodes the spike protein (S protein), a class I fusion protein that forms homotrimers protruding from the viral surface and mediates coronaviruses attachment and adhesion to human target cells via a host receptor. Spike has two different functional regions, S1 and S2. The S1 subunit consists of four core domains, S1A to S1D. The distal S1 domain mediates receptor association and stabilization, whereas the S2 domain promotes structural rearrangements and membrane fusion. Coronaviruses use different regions of S1 domain to interact with specific binding receptors. For SARS-CoV-2, the receptor binding domain (RBD) interacts with the peptidase domain of the angiotensin-converting enzyme 2 (ACE2) receptor from bats, civet cats, swine, cats, ferrets, non-human primates (NHPs), and humans (Amanat & Krammer, 2020). ACE2 is a membrane-anchored carboxypeptidase highly expressed by airway epithelial and type I and II alveolar epithelial cells (Perrotta et al., 2020).

REFERENCES

• Amanat F, Krammer F. SARS-CoV-2 Vaccines: Status Report. Immunity. 2020;52(4):583-589.
• Perrotta F, Matera MG, Cazzola M, Bianco A. Severe respiratory SARS-CoV2 infection: Does ACE2 receptor matter?. Respir Med. 2020;168:105996.