Goat Anti-4 HNE (4 Hydroxynonenal) Affinity Pure | ABMC-A22

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ABMC-A22
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  • Goat Anti-4 HNE (4 Hydroxynonenal) Affinity Pure
  • Goat Anti-4 HNE (4 Hydroxynonenal) Affinity Pure
€471.90 - €1,449.50

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Description

Goat Anti-4 HNE (4 Hydroxynonenal) Affinity Pure | ABMC-A22

Host Species: Goat
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: 4 HNE-KLH
Purification: Affinity purified
Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2
Specificity

Specifically binds to 4-HNE modified proteins. Dilution for immunoblot and ELISA range: 1,000 to 8,000. (A slight amount of precipitation may have occurred during storage due to the natural properties of these antibodies; please centrifuge before use.).

Use:

The antibody can be used for detection of of 4-HNE modification in plasma, lipoproteins, and other proteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.

Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

 

IMPORTANCE

Modifications on lysine residues, with formation of carboxylmethyl-lysine (CML), malondialdehyde (MDA) and hexitol-lysine are advanced glycation end-products (AGE), and the coupling with reactive aldehyde compounds, such as 4-hydroxynonenal (4-HNE) may appear from lipid oxidation (Guichardant et al., 1998). These modifications feature the oxidative byproducts which react with NH2 groups and form Schiff-base adducts (Mark et al., 1996). LDL treated with HNE or oxidatively modified by Cu++ or by cultured endothelial cells give rise to Michael addition-type HNE adducts that are recognized by HNE-specific antibodies.

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