aCella - AChE 1000 Tests | CLACHE100-4

aCella - AChE 1000 Tests | CLACHE100-4 | Cell Technology

Description

Key Benefits

• Safe – Non Radioactive Enzyme activity assay.
• Versatile: Nerve Gas, Pesticide Monitoring, drug screening Applications
• Homogenous – One-step, no wash assay.
• FAST – Results in 30 secs – 5 minutes.
• Highly Sensitive
• HTS – Adaptable for High Throughput format
• Applications – Standard luminometer readout

Additional information

Kit Size: 100, 500, 1000

ASSAY PRINCIPLE

We have developed a highly sensitive, very rapid, extremely simple assay for AChE activity, using the natural substrate, acetylcholine. As shown in Figure 1, a series of coupled enzyme reactions quickly translates the presence of active AChE into a change in the luminance of the reaction. First (reaction I), acetylcholine is hydrolyzed by the AChE to yield acetate and choline. The acetate and choline then enter a coupled enzyme reaction (reaction II) that results in consumption of ATP, and finally the ATP concentration is measured by the well-established luciferase method (reaction III). These reactions can occur simultaneously, and the result is generally obtained in five minutes or less. Inhibitors of AChE are readily detected by an increase in luminance due to reduced consumption of ATP.

The following reaction illustrates the sequence of events if AChE inhibitors are present:

Reaction I: AChE + Inhibitor No Acetate and Choline
Reaction II: Coupled Enzyme Reaction + ATP  Reaction does not proceed
Reaction III: ATP (remaining) + Luciferase/Luciferin  LIGHT