General Serum Diluent | ESD100-1/ESD100-2/ESD100-3/ESD100-4

General Serum Diluent | ESD100-1/ESD100-2/ESD100-3/ESD100-4 | Cell Technology

Additional information

Bottle Size: 100 mL, 500 mL, 1 L, 10 L

Improves Assays

By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.

Directions

Simply multiply the value determined from the curve by your dilution factor. For example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent) and this yielded an OD of 0.437, when read off the curve, the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL, meaning that the original starting sample had 400pg/mL of analyte present.

Diluents Available

ESD100 General-Purpose Sample Diluent – All ELISAs For dilution of most serum, ascites, cell-culture supernatants, and antigens into the functional range of the ELISA.

ESD200 Plasma Sample Diluent – Antigen-Down For dilution of plasma samples into the functional range of the antigen-down ELISA to inhibit complement and thrombin (clotting) activity during the assay.

ESD300 Picses Sample Diluent.

ECD

General-Purpose Sample Diluent is formulated for diluting goat, rabbit, mouse, and human sera, as well as cell culture media and mouse ascites fluid. ESD100 can also be used for dilution of mammalian and chicken serum samples in antigen-down ELISA formats. Hyperimmunized serum samples contain nonspecific IgG antibodies that may interfere with the titration.

Proprietary additives have been incorporated into this diluent that minimizes nonspecific binding interactions of the non-specific IgG in the samples, thus decreasing background noise.

Dilutions of the antigen standard for use in sandwich ELISA formats have also been carried out using this diluent. ESD100 contains a buffered BSA protein base to assure a constant pH and solute environment favorable to antibody-antigen interactions. It also contains an antimicrobial agent to provide a solution free of bacterial contamination.