Peroxynitrite Detection Kit | FLHPF100-2

Peroxynitrite Detection Kit | FLHPF100-2 | Cell Technology

Description

Introduction

Anew novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.

Key Benefits

• Quenched Cell permeable dye.
• One Step, No wash Homogenous assay.
• Adaptable to High throughput assay platforms.
• Can monitor multiple time points to follow real time kinetics.
• Non-destructive cell based assay allows monitoring of additional parameters.
• Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

Additional information

Kit Size: 150

ASSAY PRINCIPLE

Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003