SR Poly Caspase Kit | SR100-1/SR100-2

SR Poly Caspase Kit | SR100-1/SR100-2 | Cell Technology

Description

Key Benefits

• Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
• Cell permeablity permits direct visualization of cytosolic apoptotic events.
• Apoptotic cell population does not diminish over time.
• Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
• Works in diverse cell lines: human, rodent, Drosophila.
• Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
• Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
• Applications – Works with fluorescence microscope, 96-well fluorescence plate readers
• Yields both quantitative and qualitative results. Gives strong signal with little background noise.

Additional information

Kit Size: 25, 100

ASSAY PRINCIPLE

APO LOGIX SR kits contain a generic sulforhodamine labeled caspase inhibitor (sulforhodamine-peptide-fluoromethyl ketone). This reagent is cell permeable and is used on whole cells to detect apoptosis. Apoptotic cells are detected by a fluorescence plate reader or fluorescence microscope using an excitation source at 550nm and measuring emission at 595nm. The assay takes about 1 hr to complete
APO LOGIX Sulforhodamine

Jurkat cells stimulated with staurosporine for 2 hours and then labeled with SR-VAD-FMK.

Left side: 30X phase contrast

Right side: 30X fluorescence microscope. Excitation: 550nm emission > 580nm.
APO LOGIX Sulforhodamine

Jurkat cells stimulated with staurosporine for 2 hours. Cells were then stained with SR-VAD-FMK for 1 hour and read in a 96 well fluorescence plate reader.