Anti-Mouse Alpha 5 (IV) NC1 Antibody, Clone M54

Anti-Mouse Alpha 5 (IV) NC1 Antibody, Clone M54 - Cat Number: 7079 From Chondrex.

Research Field: Nephritis, ECM

Clonality: Monoclonal

Cross-Reactivity: Ms

Host Origin: Rat

Applications: IHC, WB

Isotype: IgG2a

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen: Mouse Alpha 5 (Type IV Collagen) NC1

PRODUCT SPECIFICATIONS

DESCRIPTION: Rat IgG2a monoclonal antibody against the mouse non-collagenous domain 1 (NC1) of the
alpha 5 chain of type IV collagen (Alpha 5 (IV) NC1), clone M54, unconjugated
APPLICATION: Use for immunohistochemistry* and immunoblotting
* Acid treatment is required for immunohistochemistry - Soak tissue sections on the slide in 6M
urea, 0.1M glycine-HCl buffer, pH 3.5, for 30 minutes; then rinse three times with PBS
QUANTITY: 0.1 ml, 1 mg/ml
FORM: Solution in 0.05M Phosphate Buffered Saline, pH 7.4
SOURCE: Rat
REACTIVITY: Reacts to mouse Alpha 5 (IV) NC1
IMMUNOGEN: Peptide: ATVDMSDMFNKPQSETLC
PURITY: Purified by Protein G chromatography
SUBTYPE: Rat IgG2a
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent nonspecific reactions in ELISA and tissue staining.
STORAGE: -20⁰C
STABILITY: 1 year
NOTES: N/A
REFERENCES: M. Kagawa, Y. Kishiro, I. Naito, T. Nemoto, H. Nakanishi, Y. Ninomiya, Y. Sado. Nephrol Dial
Transplant.12(6):1238-41 (1997).
Y. Sado, M. Kagawa, Y. Kishiro, K. Sugihara, I. Naito, J. Seyer, M. Sugimoto, T. Oohashi, Y.
Ninomiya, Histochem Cell Biol 104:267-75 (1995).
I. Naito, S. Kawai, S. Nomura, Y. Sado, G. Osawa, Kidney Int 50:304-11 (1996).