Description
Anti-Type II Collagen (CB11) Antibody, Clone A2-10 - Cat Number: 7050 From Chondrex.
Research Field: Arthritis, ECM
Clonality: Monoclonal
Cross-Reactivity: B, C, H, Mk, Ms, P, R
Host Origin: Mouse
Applications: ELISA, IHC, WB
Isotype: IgG2z
Detection Range: N/A
Sample Type: N/A
Concentration: 1 mg/ml
Immunogen: Type II Collagen (CB11)
PRODUCT SPECIFICATIONS
DESCRIPTION: Mouse Anti-Chick Type II Collagen (CB11) IgG2a Monoclonal Antibody, Clone 2A-10,
unconjugated.
Clone A2-10 recognizes the conserved epitopes on various species of type II collagen.
APPLICATION: Use for immunohistochemistry, immunoblotting, and ELISA
QUANTITY: 0.1 ml, 1 mg/ml
FORM: Solution in 0.05M Phosphate Buffered Saline, pH 7.4
SOURCE: Mouse
REACTIVITY: Cross-reacts (by more than 95%) to most species of type II collagen including mouse, porcine,
chick, bovine, human, rat, and monkey.
IMMUNOGEN: Chick Type II Collagen
PURITY: Purified by Protein G chromatography
SUBTYPE: Mouse IgG2a
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent nonspecific reactions in ELISA and tissue staining. Working concentration: 0.1 - 1 µg/ml. If detecting
denatured collagen, a 10X higher concentration of cocktail will be required for immunoblotting
due to low reactivity to denatured collagen.
STORAGE: -20⁰C
STABILITY: 1 year
NOTES: N/A
REFERENCES: Terato K. et al. J. Immunol. 148: 2103-2108 (1992)
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