Anti-Type II Collagen (CB11) Antibody, Clone D1-2G

Anti-Type II Collagen (CB11) Antibody, Clone D1-2G - Cat Number: 7052 From Chondrex.

Research Field: Arthritis, ECM

Clonality: Monoclonal

Cross-Reactivity: B, C, H, Mk, Ms, P, R

Host Origin: Mouse

Applications: ELISA, IHC, WB

Isotype: IgG2b

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen: Type II Collagen (CB11)

PRODUCT SPECIFICATIONS

DESCRIPTION: Mouse Anti-Chick Type II Collagen (CB11) IgG2b Monoclonal Antibody, Clone D1-2G,
unconjugated
APPLICATION: Use for immunohistochemistry, immunoblotting, and ELISA
QUANTITY: 0.1 ml, 1 mg/ml
FORM: Solution in 0.05M Phosphate Buffered Saline, pH 7.4
SOURCE: Mouse
REACTIVITY: Cross-reacts to most species of type II collagen including mouse, porcine, chick, bovine, human,
rat, monkey, rabbit, equine, and dog. Cross-reactivity with denatured type II collagen is minimal
(less than 10 %).
Epitope: LyC1 of CB11 on Type II collagen
IMMUNOGEN: Chick Type II Collagen
PURITY: Purified by Protein G chromatography
SUBTYPE: Mouse IgG2b
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent nonspecific reactions in ELISA and tissue staining. Working concentration: 0.1 - 1 µg/ml. If detecting
denatured collagen, a 10X higher concentration of cocktail will be required for immunoblotting
due to low reactivity to denatured collagen.
STORAGE: -20⁰C
STABILITY: 1 year
NOTES: N/A
REFERENCES: K. Terato, K. Hasty, R. Reife, M. Cremer, A. Kang, J. Stuart, et al., Induction of arthritis with
monoclonal antibodies to collagen. J Immunol 148, 2103-8 (1992).
P. Hutamekalin, T. Saito, K. Yamaki, N. Mizutani, D. Brand, et al., Collagen antibody-induced
arthritis in mice: development of a new arthritogenic 5-clone cocktail of monoclonal anti-type II
collagen antibodies. J Immunol Methods 343, 49-55 (2009).