Anti-Type II Collagen (CB11) Antibody Cocktail (A2-10, D1-2G, D8-6, F10-21), Biotinylated

Anti-Type II Collagen (CB11) Antibody Cocktail (A2-10, D1-2G, D8-6, F10-21), Biotinylated - Cat Number: 7006 From Chondrex.

Research Field: Arthritis, ECM

Clonality: Monoclonal

Cross-Reactivity: B, C, H, Mk, Ms, P, R

Host Origin: Mouse

Applications: ELISA, IHC, WB

Isotype: IgG2a/2b

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen: Type II Collagen (CB11)

PRODUCT SPECIFICATIONS

DESCRIPTION: Mouse Anti-Type II Collagen Monoclonal Antibody Cocktail, biotinylated
A cocktail of 4 monoclonal antibodies, clone A2-10 (IgG2a), F10-21 (IgG2a), D8-6 (IgG2a) and
D1-2G (IgG2b), recognizing the conserved epitopes on various species of type II collagen.
APPLICATION: Use for immunohistochemistry, immunoblotting, and ELISA
QUANTITY: 0.1 ml, 1 mg/ml
FORM: Solution in buffered normal goat serum, pH 7.2
SOURCE: Mouse
REACTIVITY: Cross-reacts (by more than 95%) to most species of type II collagen including mouse, porcine,
chick, bovine, human, rat, monkey, rabbit, equine and dog.
IMMUNOGEN: Chick Type II Collagen
PURITY: Purified by Protein G chromatography
SUBTYPE: Mouse IgG2a and IgG2b
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent nonspecific reactions in ELISA and tissue staining. Working concentration: 0.1 - 1 µg/ml. If detecting
denatured collagen, a 10X higher concentration of cocktail will be required for immunoblotting
due to low reactivity to denatured collagen.
STORAGE: -20⁰C
STABILITY: 1 year
NOTES: N/A
REFERENCES: Terato K. et al. J. Immunol. 148: 2103-2108 (1992)