Mouse IgE Monoclonal Antibody, Isotype Control, Clone 2A101A12

Mouse IgE Monoclonal Antibody, Isotype Control, Clone 2A101A12 - Cat Number: 7129 From Chondrex.

Research Field: Allergy

Clonality: Monoclonal

Cross-Reactivity: Unknown

Host Origin: Mouse

Applications: ELISA, WB, Cell Based Assays

Isotype: IgE

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen:

DESCRIPTION: Mouse IgE Monoclonal Antibody, Isotype Control, Clone 2A101A12, unconjugated

Antibody characterization studies, such as inhibition of target proteins in in-vivo or in-vitro
studies, must use an appropriate isotype control antibody matched to the class and type of the
test antibody in order to properly interpret the results. Many researchers use the buffer that
dissolved the test antibody as a negative control, however this is not a sufficient control.

Because this isotype control has no relevant function, it works as a negative control to effectively
distinguish non-specific background reactions from specific antibody reactions. Non-specific
reactions are caused by 1) Fc receptors on cells, 2) antibody interaction with cellular proteins,
lipids, or carbohydrates, and 3) cellular autofluorescence.

Clone 2A101A12 (BALB/c IgE,κ) for use as an IgE isotype control in in-vivo and in-vitro studies.
APPLICATION: Use for in-vivo studies and in-vitro studies such as ELISA, Western blotting, and cell-based
assays QUANTITY: 0.5 ml, 1 mg/ml
FORM: Solution in 0.05M phosphate buffered saline, pH 7.4
SOURCE: Mouse
REACTIVITY: N/A
IMMUNOGEN: N/A
PURITY: Purified by ion-exchange chromatography SUBTYPE: Mouse IgE
USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as normal goat serum, to
prevent non-specific reactions in ELISA.
STORAGE: -20°C
STABILITY: 1 year
NOTES: N/A
REFERENCES: N/A