Nephritogenic Monoclonal Antibody, Clone 114, 5 mg

Nephritogenic Monoclonal Antibody, Clone 114, 5 mg - Cat Number: 70205 From Chondrex.

Research Field: Nephritis

Clonality: Monoclonal

Cross-Reactivity:

Host Origin: Mouse

Applications: N/A

Isotype: IgG1

Detection Range: N/A

Sample Type: N/A

Concentration: 1 mg/ml

Immunogen:

DESCRIPTION: Rat monoclonal antibody which recognizes rat non-collagenous domain 1 of the alpha 4 chain of type IV collagen (alpha 4 (IV) NC1), clone a84, unconjugated

APPLICATION: Use for developing rat nephritis models, immunohistochemistry, and immunoblotting
QUANTITY: 1 ml x 1 mg/ml (Cat # 70211)
5 ml x 1 mg/ml (Cat # 70215)
FORM: Solution dissolved in 0.05M phosphate buffered saline, pH 7.4
SOURCE: Rat
IMMUNOGEN: Alpha chain NC1 domains of Rat Type IV Collagen
REACTIVITY: Reacts to rat alpha 4 (IV) NC1
SUBTYPE: Rat IgG2a
USAGE: In vivo studies: please refer to Chondrex Inc’ s Rat Nephritogenic Monoclonal Antibodies to Glomerular Basement Membrane protocol for inducing nephritis in WKY rats.
In vitro studies: dilute the antibody in buffer containing 2% heterologous serum, such as normal
goat serum, to prevent non-specific reactions in ELISA, tissue staining, or immunoblotting.
STORAGE: -80°C
STABILITY: 1 year
NOTES: N/A
REFERENCES: T. Kohda, S. Okada, A. Hayashi, S. Kanzaki, Y. Ninomiya, et al., High nephritogenicity of monoclonal antibodies belonging to IgG2a and IgG2b subclasses in rat anti-GBM nephritis.
Kidney Int 66, 177-86 (2004).