Human Type I Collagen Detection Kit

Human Type I Collagen Detection Kit - Cat Number: 6021 From Chondrex.

Research Field: ECM

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 5 µg/ml-0.08 µg/ml

Sample Type: Culture Media, Solubilized Collagen

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: ELISA kits to quantify Type I Collagen
6012: Mouse Type I Collagen Detection ELISA Kit
6013: Rat Type I Collagen Detection ELISA Kit
6014: Bovine Type I Collagen Detection ELISA Kit
6015: Porcine Type I Collagen Detection ELISA Kit
6016: Rabbit Type I Collagen Detection ELISA Kit
6019: Canine Type I Collagen Detection ELISA Kit
6021: Human Type I Collagen Detection ELISA Kit
FORMAT: 96-well ELISA plate with removeable strips
ASSAY TYPE: Sandwich ELISA
ASSAY TIME: 5.5 hours
STANDARD RANGE: 6012/6013/6015/6021: 5 µg/ml to 0.08 µg/ml
6014/6016/6019: 10 µg/ml to 0.16 µg/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Solubilized collagen and cell culture media
RECOMMENDED SAMPLE DILUTIONS: 1:1 - 1:1000
CHROMOGEN: OPD (read at 490 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA: 6021 (only): Intra-assay (1.4-3.9%)/Inter-assay (2-6.6%)/Spiking Test (99-106%)
NOTES: These kits are intended to detect native type I collagen and will only weakly detect denatured
collagen

INTRODUCTION

Type I collagen is one of several interstitial fibrillar collagens consisting of two identical α1(I) chains and one α2(I) chain. It is the most
abundant collagen type and is found in most connective tissues such as skin, bone, tendon, ligament, and heart. Many tissues contain
heterotypic fibrils meaning that two or more distinct collagen types coexist. For example, most connective tissues (except for bone) contain
heterotypic fibrils of type I and III collagen, although the type III collagen content is minor compared to Type I collagen. Chondrex, Inc.
provides a variety of species-specific Type I Collagen Detection ELISA Kits designed to quantify the amount of species-specific type I collagen
in cultured cells and tissue specimens.

ANTIBODY SPECIFICITY

Monoclonal antibodies are used as the capture and detection antibodies in these Type I Collagen Detection ELISA Kits. All clones are highly
specific to the native conformation of their respective type I collagens and poorly cross-react with denatured type I collagen. Chondrex, Inc.
recommends carefully selecting kits to determine species-specific type I collagen in samples containing other species of type I collagen or
type II collagen based on the specificity of the capture antibodies in the table below. Some capture antibodies are highly specific to their
respective type I collagens and do not cross-react with other species of type I or type II collagen.

KIT COMPONENTS 

ASSAY OUTLINE

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