Mouse Albumin Detection Kit

Mouse Albumin Detection Kit - Cat Number: 3012 From Chondrex.

Research Field: Nephritis

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 100 ng/ml-1.6 ng/ml

Sample Type: Serum, Plasma, Urine

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: ELISA Kit to quantify mouse albumin
FORMAT: 96-well ELISA Plate with removeable strips
ASSAY TYPE: Sandwich ELISA
ASSAY TIME: 4 hours
STANDARD RANGE: 100 - 1.6 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Urine and Serum
RECOMMENDED SAMPLE DILUTIONS: 1:500 (at least)
CHROMOGEN: OPD (read at 490 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA: Intra-Assay (1.6-1.9%)/Inter-Assay (1.7-2.6%)/Spiking Test (89-98%)
NOTES

INTRODUCTION

Proteinurea is a common symptom of nephritis in humans and experimental animals and is an important marker for evaluating disease severity regardless of the type of nephritis. Moreover, proteinurea is determined as the total amount of proteins (serum proteins such as globulins and albumin) excreted into a 16-hour period, which is distinctly more accurate than determining the urinary protein concentration, due to large variations in urine volume between individual animals. The turbidity method is a commonly used urinary protein assay method because it is accurate, easy, and economical.

However, mouse kidneys leak serum components such as bilirubin, resulting in overestimated urinary protein levels, thus proteinuria is not a suitable marker for the evaluation of nephritis severity. On the other hand, albumin, a serum protein with a relatively small molecular weight, and typically the first protein observed in the urine when kidney dysfunction begins to develop, is a more suitable marker to evaluate the severity of nephritis in mouse models.

Chondrex, Inc.’s Mouse Urinary Albumin Detection ELISA Kit (Cat # 3012) is designed to specifically determine mouse albumin in 40 urine samples within 4 hours using a sandwich immunoassay method. In mouse nephritis models, such as immune complex-induced glomerulonephritis (ICGN), a mouse is deemed nephritic if the total albumin content in a 16-hour urine collection is greater than 1 mg. It is important to note that urinary albumin levels can reach up to 50-200 mg in a 16-hour urine collection, depending on the severity of nephritis.

NOTES BEFORE USING ASSAY
NOTE 1: It is recommended that the standard and samples be run in duplicate.
NOTE 2: Warm up all buffers to room temperature before use.
NOTE 3: Crystals may form in Wash Buffer, 20X when stored at cold temperatures. If crystals have formed, warm the wash buffer by placing the bottle in warm water until crystals are completely dissolved.
NOTE 4: Measure exact volume of buffers using a serological pipet, as extra buffer is provided.
NOTE 5: Cover the plate with plastic wrap or a plate sealer after each step to prevent evaporation from the outside wells of the plate.
NOTE 6: For partial reagent use, please see the assay protocol’s corresponding step for the appropriate dilution ratio. For example, if the protocol dilutes 50 µl of a stock solution in 10 ml of buffer for 12 strips, then for 6 strips, dilute 25 µl of the stock solution in 5 ml of buffer. Partially used stock reagents may be kept in their original vials and stored at -20⁰C for use in a future assay.
NOTE 7: This kit contains animal components from non-infectious animals and should be treated as potential biohazards in use and for disposal.