Mouse Anti-ssDNA IgM Antibody Assay Kit

Mouse Anti-ssDNA IgM Antibody Assay Kit - Cat Number: 3042 From Chondrex.

Research Field: Systemic Lupus Erythematosus

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 1000 ng/ml-16 ng/ml

Sample Type: Serum, Plasma

Concentration: N/A

Immunogen:

DESCRIPTION: ELISA kits to quantify mouse IgM antibodies against single-stranded DNA (ssDNA) and
double-stranded DNA (dsDNA).

3032: Mouse Anti-dsDNA IgM Antibody ELISA Kit
3042: Mouse Anti-ssDNA IgM Antibody ELISA Kit
FORMAT: 96-well ELISA plate with removeable strips
ASSAY TYPE: Indirect ELISA
ASSAY TIME: 4.5 hours
STANDARD RANGE: 50 ng/ml to 0.8 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum & Plasma (pre-treatment acceptable)
RECOMMENDED SAMPLE DILUTIONS: 1:100 or greater
CHROMOGEN: TMB (read at 450 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA: 3032: Intra-Assay (0.1-8.1%)/Inter-Assay (6.0-8.6%)/Spiking Test (92.9-98.3%)
3042: Intra-Assay (2.7-6.3%)/Inter-Assay (2.7-7.0%)/Spiking Test (96.4-101.3%)

INTRODUCTION

High levels of serum autoantibodies against deoxyribonucleic acid (DNA) are observed in most patients with systemic lupus erythematosus (SLE) (1, 2), therefore the presence of anti-DNA antibodies in serum is considered a valuable marker for the diagnosis of SLE. Especially considering that the serum anti-DNA antibodies form anti-DNA/DNA immune complexes which play important roles in the immunopathogenesis of renal injury also known as lupus nephritis (3).

With regards to the specificity of anti-DNA antibodies, anti-single stranded DNA (ssDNA) lgG antibodies are elicited in the early stages of SLE, whereas anti-double stranded DNA (dsDNA) lgG antibody levels correlate with the severity of SLE. On the other hand, anti-dsDNA lgM antibodies are not specific to SLE, but correlate with the prognosis of lupus nephritis in patients with SLE (4, 5). Therefore, evaluating immunoglobulin levels of different isotypes against individual DNA types may indicate the stages and prognosis of SLE. Mouse models, which provide relevant information to the human condition, elucidate the cellular and genetic requirements for inducing SLE.

For example, in spontaneous murine NZB/W F1 lupus models, anti-dsDNA antibody isotype class switching from lgM to lgG indicates renal failure which is a similar trend in human SLE (6). Nonetheless, in artificial pristane-induced Balb/c lupus models, anti-ssDNA lgM antibodies solely induce SLE (7-9). In order to advance studies in these fields, Chondrex, Inc. provides mouse anti-DNA antibody ELISA kits to elucidate the diverse roles of anti-DNA antibodies in these mouse SLE models. For a complete list of available mouse anti-DNA antibody ELISA kits, please visit www.chondrex.com for more information.

KIT COMPONENTS

ASSAY OUTLINE

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