Mouse Total IgM Antibody Detection Kit

Mouse Total IgM Antibody Detection Kit - Cat Number: 3024 From Chondrex.

Research Field: Immunology

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 100 ng/ml-1.6 ng/ml

Sample Type: Serum, Plasma

Concentration: N/A

Immunogen:

DESCRIPTION: ELISA kits to quantify total mouse IgG/IgM/IgA immunoglobulins.

FORMAT: 96-well ELISA plate with removeable strips
ASSAY TYPE: Sandwich ELISA
ASSAY TIME: 4.5 hours
STANDARD RANGE: 100 ng/ml to 1.6 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum & Plasma (pre-treatment acceptable)
RECOMMENDED SAMPLE DILUTIONS: Varies (Starting point 1:100 or greater)
CHROMOGEN: OPD (read at 490 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA: 3019 (IgA): Intra-Assay (3.2-7.9%)/Inter-Assay (3.3-8.5%)/Spiking Test (102-104%)
3023 (IgG): Intra-Assay (4.0-10.1%)/Inter-Assay (4.2-5.7%)/Spiking Test (93-113%)
3025 (IgG1): Intra-Assay (1.6-8.0%)/Inter-Assay (2.8-7.1%)/Spiking Test (93-100%)
3026 (IgG2a): Intra-Assay (8.3-9.0%)/Inter-Assay (4.1-6.9%)/Spiking Test (84-102%)
3027 (IgG2b): Intra-Assay (4.1-7.5%)/Inter-Assay (5.2-9.1%)/Spiking Test (93-100%)
3028 (IgG3): Intra-Assay (0.9-9.7%)/Inter-Assay (0.8-3.6%)/Spiking Test (86-97%)
3024 (IgM): Intra-Assay (2.3-6.5%)/Inter-Assay (2.7-5.1%)/Spiking Test (94-112%)
NOTES: N/A

INTRODUCTION

Animals are consistently exposed to environmental factors that penetrate the body from multiple sites, such as the respiratory and digestive tracts, or through damaged skin. Penetrating antigens can activate the humoral immune response which produces anti-antigen immunoglobulins to protect the host. Immunoglobulins are classified by their isotypes which differ by biological properties, functional locations, and ability to deal with different antigens. The main isotypes are summarized below: 1) Immunoglobulin A (IgA) plays a critical role in mucosal immunity and is found in the mucous membranes, especially the respiratory and gastrointestinal tracts, as well as in saliva and tears. 2) Immunoglobulin E (IgE) is involved in allergic reactions and is found in the lungs, skin, and mucous membranes.

3) Immunoglobulin G (IgG) is the most abundant isotype and is found in blood and extracellular fluids. In mice, IgG is categorized into four subtypes: IgG1, IgG2a, IgG2b, and IgG3. 4) Immunoglobulin M (IgM) is the first isotype produced in a humoral immune response and is found mainly in the blood and lymph fluid. Total immunoglobulin isotype levels correlate with health or pathological conditions such as hypo- or hyper-gammaglobulinemia, and acute or chronic infections (1-5). In addition, total immunoglobulin levels are often analyzed relative to antigen-specific antibody levels to evaluate immune function in patients and animal disease models.

Chondrex, Inc. provides mouse total immunoglobulin isotype ELISA kits for IgA, IgE, IgM, and IgG, as well as IgG subtypes: IgG1, IgG2a, IgG2b, and IgG3, along with antigen-specific antibody ELISA kits, such as autoantigens (ssDNA and dsDNA), dietary proteins (collagen and OVA), and bacteria (E. coli, LPS and S. aureus). These total immunoglobulin detection ELISA kits are also suitable for quantifying monoclonal antibody and polyclonal antibody isotypes and/or subtypes, as well as establishing reliable methods for monitoring antibody production and quality control. 

LIST OF MOUSE TOTAL IMMUNOGLOBULIN DETECTION ELISA KITS

KIT COMPONENTS

PLATE MAPPING

ASSAY OUTLINE

NOTES BEFORE USING ASSAY

NOTE 1: It is recommended that the standard and samples be run in duplicate.
NOTE 2: Warm up all buffers to room temperature before use.
NOTE 3: Crystals may form in Wash Buffer, 20X when stored at cold temperatures. If crystals have formed, warm the wash buffer by placing the bottle in warm water until crystals are completely dissolved.
NOTE 4: Measure exact volume of buffers using a serological pipet, as extra buffer is provided.
NOTE 5: Cover the plate with plastic wrap or a plate sealer after each step to prevent evaporation from the outside wells of the plate.
NOTE 6: For partial reagent use, please see the assay protocol’s corresponding step for the appropriate dilution ratio. For example, if the protocol dilutes 50 µl of a stock solution in 10 ml of buffer for 12 strips, then for 6 strips, dilute 25 µl of the stock solution in 5 ml of buffer. Partially used stock reagents may be kept in their original vials and stored at -20⁰C for use in a future assay.
NOTE 7: This kit contains animal components from non-infectious animals and should be treated as potential biohazards in use and for disposal.