Rat Anti-Porcine Type II Collagen IgG Antibody Assay Kit, OPD

Rat Anti-Porcine Type II Collagen IgG Antibody Assay Kit, OPD - Cat Number: 2043 From Chondrex.

Research Field: Arthritis, Immunology

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 16 units/ml-0.25 units/ml

Sample Type: Serum, Plasma

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Assay kit to quantify rat anti-collagen antibodies
FORMAT: Pre-coated 96-well ELISA Plate with removeable strips
ASSAY TYPE: Indirect ELISA
ASSAY TIME: 3.5 hours
STANDARD RANGE: 16 units/ml to 0.25 units/ml
NUMBER OF SAMPLES: Up to 39 (duplicate) samples/standard plate (will vary for custom kits)
SAMPLE TYPES: Serum and Plasma
RECOMMENDED SAMPLE DILUTIONS: 1:1000 (at least)
CHROMOGEN: OPD (read at 490 nm)
STORAGE: -20°C
VALIDATION DATA: N/A
NOTES: This kit has an overnight incubation step

INTRODUCTION

Heterologous type II collagen is widely used as an immunogen for the collagen-induced arthritis (CIA) model. In CIA-susceptible rats, the serum antibody levels to the type II collagen used for immunization are very high. Furthermore, these antibodies cross-react to various species of type II collagen including autologous type II collagen, due to the conserved amino acid sequences of type II collagen. It is important to note that although type I collagen shares about 80% of its amino acid sequence with type II collagen, it is not capable of inducing autoimmune-mediated diseases. This indicates that epitope specificity of antibodies and T-cells are important for establishing autoimmunity and subsequent development of autoimmune diseases. Therefore, type I collagen might be useful as a control for studying the pathogenesis of autoimmune mediated arthritis and B and T-cell epitope specificity.

This kit is designed to assay type I and type II collagen antibodies in rat serum. Chondrex Inc.’s ELISA systems incorporate unique blocking agents to reduce non-specific reactions. These agents reduce the background levels by inhibiting the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces. Chondrex, Inc. offers various species of type I and type II collagen-coated strips as shown below.