Rat Type I Collagen Detection Kit

Rat Type I Collagen Detection Kit - Cat Number: 6013 From Chondrex.

Research Field: ECM

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 5 µg/ml-0.08 µg/ml

Sample Type: Culture Media, Solubilized Collagen

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: ELISA kits to quantify Type I Collagen
6012: Mouse Type I Collagen Detection ELISA Kit
6013: Rat Type I Collagen Detection ELISA Kit
6014: Bovine Type I Collagen Detection ELISA Kit
6015: Porcine Type I Collagen Detection ELISA Kit
6016: Rabbit Type I Collagen Detection ELISA Kit
6019: Canine Type I Collagen Detection ELISA Kit
6021: Human Type I Collagen Detection ELISA Kit
FORMAT: 96-well ELISA plate with removeable strips
ASSAY TYPE: Sandwich ELISA
ASSAY TIME: 5.5 hours
STANDARD RANGE: 6012/6013/6015/6021: 5 µg/ml to 0.08 µg/ml
6014/6016/6019: 10 µg/ml to 0.16 µg/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Solubilized collagen and cell culture media
RECOMMENDED SAMPLE DILUTIONS: 1:1 - 1:1000
CHROMOGEN: OPD (read at 490 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA: 6021 (only): Intra-assay (1.4-3.9%)/Inter-assay (2-6.6%)/Spiking Test (99-106%)
NOTES: These kits are intended to detect native type I collagen and will only weakly detect denatured
collagen

INTRODUCTION

Type I collagen is one of several interstitial fibrillar collagens consisting of two identical α1(I) chains and one α2(I) chain. It is the most abundant collagen type and is found in most connective tissues such as skin, bone, tendon, ligament, and heart. Many tissues contain heterotypic fibrils meaning that two or more distinct collagen types coexist. For example, most connective tissues (except for bone) contain heterotypic fibrils of type I and III collagen, although the type III collagen content is minor compared to Type I collagen. Chondrex, Inc. provides a variety of species-specific Type I Collagen Detection ELISA Kits designed to quantify the amount of species-specific type I collagen in cultured cells and tissue specimens.

ANTIBODY SPECIFICITY

Monoclonal antibodies are used as the capture and detection antibodies in these Type I Collagen Detection ELISA Kits. All clones are highly specific to the native conformation of their respective type I collagens and poorly cross-react with denatured type I collagen. Chondrex, Inc. recommends carefully selecting kits to determine species-specific type I collagen in samples containing other species of type I collagen or type II collagen based on the specificity of the capture antibodies in the table below. Some capture antibodies are highly specific to their respective type I collagens and do not cross-react with other species of type I or type II collagen

NOTES BEFORE USING ASSAY
NOTE 1: It is recommended that the standard and samples be run in duplicate.
NOTE 2: Warm up all buffers to room temperature before use.
NOTE 3: Crystals may form in Wash Buffer, 20X when stored at cold temperatures. If crystals have formed, warm the wash buffer by placing
the bottle in warm water until crystals are completely dissolved.
NOTE 4: Measure exact volume of buffers using a serological pipet, as extra buffer is provided.
NOTE 5: Cover the plate with plastic wrap or a plate sealer after each step to prevent evaporation from the outside wells of the plate.
NOTE 6: For partial reagent use, please see the assay protocol’s corresponding step for the appropriate dilution ratio. For example, if the
protocol dilutes 50 µl of a stock solution in 10 ml of buffer for 12 strips, then for 6 strips, dilute 25 µl of the stock solution in 5 ml of buffer.
Partially used stock reagents may be kept in their original vials and stored at -20⁰C for use in a future assay.
NOTE 7: This kit contains animal components from non-infectious animals and should be treated as potential biohazards in use and for disposal.