Cell Culture Grade Bovine Type I Collagen, 4 mg/ml x 12.5 ml

Cell Culture Grade Bovine Type I Collagen, 4 mg/ml x 12.5 ml - Cat Number: 1202 From Chondrex.

Research Field: Cell Culture, Bioengineering

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: 4 mg/ml

Immunogen:

PRODUCT SPECIFICATIONS

Collagen, the most abundant protein in vertebrates, is observed in skin, cartilage, bone, intervertebral discs, blood vessels, tendons,
ligaments, and corneas, and is the main component of the extracellular matrix (ECM). Type I collagen consists of two identical alpha 1 chains
and one distinct alpha 2 chain (1), forming a triple helix formation known as tropocollagen. This triple-stranded helical conformation increases
structural strength and resistance to enzymatic degradation in tissue and plays a key role in assembling the ECM.
Collagen is useful for facilitating tissue regeneration and/or site-specific drug delivery (2) because of its properties such as low antigenicity,
low toxicity, high water solubility, and high biodegradability. In particular, type I collagen binds integrins (3,4) facilitating cell migration (5),
attachment (4,6), and proliferation and differentiation (3,6). Although atelocollagen, in which the telopeptides on the N- and C-terminals are
removed from tropocollagen by pepsin digestion, is widely used in industrial purposes, tropocollagen may be beneficial as the native collagen
scaffold containing cross-linked telopeptides.
Chondrex, Inc. provides an acid soluble type I tropocollagen solution which can be used for traditional two-dimensional (2D) systems as well
as a scaffold in three dimensional (3D) gels for simulating cell growth in fibroblasts (7,8) and chondrocytes (9). To determine the individual
types of collagen, please inquire about Chondrex, Inc.’s “Tips for Collagen Solubilization” protocols, as well as ELISA detection kits for type
I collagen and type II collagen. Please visit www.chondrex.com or contact support@chondrex.com for more details
DESCRIPTION: Acid soluble bovine type I collagen solution
APPLICATION: Use for two-dimensional (2D) systems as well as a scaffold in three dimensional (3D) gels for
simulating cell growth in fibroblasts and chondrocytes.
QUANTITY: 4 mg/ml x 12.5 ml (sterile filtered)
FORM: Solution in 0.01M HCl
SOURCE: Bovine
ENDOTOXIN: Less than 10 EU/ml
PURITY: >95% Type I collagen
STORAGE: 4°C
STABILITY: 6 months
PROTOCOL: PLATE COATING (NOTE: an optimized coating condition is required for your culture system)
1. Dilute the 4 mg/ml collagen with 0.02M HCl at 50 to 100 μg/ml.
2. Gently mix the diluted solution.
3. Add an appropriate volume of diluted collagen solution into wells or plates.
NOTE: Ensure the entire surface is coated.

4. Incubate at room temperature or 37°C for 1-2 hours.
5. Remove all solution.
6. Rinse coated surfaces carefully with culture media or PBS.
7. The coated well or plates can be stored at 2-8°C or air dried if sterility is maintained.
THREE-DIMENSIONAL (3D) GEL PREPARATION PROCEDURES
1. Dilute the collagen solution with equal volume of cold sterilized PBS (final 2 mg/ml).
2. Add an appropriate volume of the diluted collagen solution in wells or plates.
3. Incubate at 37°C for 30-60 minutes.
4. The gel can be stored at 2-8°C or dried if sterility is maintained.
NOTES: N/A
REFERENCES:
1. M. Shoulders, R. Raines, Collagen structure and stability. Annu Rev Biochem 78, 929-58
(2009).
2. W. Friess, Collagen--b