Chick CB8 Peptide of Type II Collagen, 0.5 mg, lyophilized

Chick CB8 Peptide of Type II Collagen, 0.5 mg, lyophilized - Cat Number: 9063 From Chondrex.

Research Field: Arthritis

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Cyanogen bromide cleaved peptide 8 (CB8) of chick type II collagen
APPLICATION: Use as an antigen to stimulate T-cells for T-cell proliferation assays and as an ELISA antigen
for assaying antibodies to type II collagen.
NOTE: For assaying antibodies that react to native type II collagen using this peptide fragment,
renature this peptide by stepwise cooling according to the method described by Igarashi et al
(1) and Terato et al (2).
QUANTITY: 0.5 mg
FORM: Lyophilized powder
SOURCE: Chick Type II Collagen
MOLECULAR WEIGHT: Approximately 14 kDa (a peptide representing residues 402 - 551 of type II collagen) (3)
PURITY: >95% by 10% SDS-PAGE
STORAGE: 4°C in the dark for lyophilized form and -20°C for solution form. This product is stable for 2
years as supplied. Peptide fragments will gradually degrade under neutral conditions.
STABILITY: 2 years
NOTES: Soluble in 0.05M acetic acid, neutral buffers containing 0.15M NaCl, and cell culture medium
REFERENCES:
1. S. Igarashi, A. Kang, J. Gross, Biochem. Biophyis. Res. Comm. 38: 697-701 (1970)
2. K. Terato, M. Cremer, K. Hasty, et al. Collagen Relat. Res. 5: 469-480 (1985)
3. E. Miller and L. Lunde, Biochemistry 12: 3153-3159 (1973)