Immunization Grade Porcine Type II Collagen, 2 mg/ml x 5ml

Immunization Grade Porcine Type II Collagen, 2 mg/ml x 5ml - Cat Number: 20032 From Chondrex.

Research Field: Arthritis

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: 2 mg/ml

Immunogen:

DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose, and Na2HPO4)
APPLICATION: Can be used for various purposes, such as a standard for collagen analysis by SDS-gel,
substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for the
induction of collagen-induced arthritis (CIA) in certain species and strains of experimental
animals.
QUANTITY: 5 ml
FORM: 2 mg/ml solution in 0.05M acetic acid
SOURCE: Porcine articular cartilage
MOLECULAR WEIGHT: 300 kDa
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
STORAGE: -20°C in the dark
STABILITY: 2 years
NOTES: The maximum concentration of type II collagen in acidic buffer is 4 mg/ml. In order to neutralize
this product, add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (NaCl
final concentration: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.
NOTE: Although type II collagen does not form gel at low concentrations, Chondrex, Inc.
recommends keeping the collagen solution on ice.
REFERENCES: R. Trelstad, et al. Biochemistry 9: 4993-4998 (1970)
E. Miller, Biochemistry 10: 1652-1659 (1971)
D. Trentham, et al. J. Exp. Med. 146: 857-868 (1977)
P. Wooley, et al. J. Immunology 135: 2443-2451 (1985)