NC1 Fragment of Bovine GBM Type IV Collagen, 1 mg, lyophilized

NC1 Fragment of Bovine GBM Type IV Collagen, 1 mg, lyophilized - Cat Number: 1102 From Chondrex.

Research Field: Nephritis

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

DESCRIPTION: NC1 fraction of type IV collagen isolated from collagenase-solubilized bovine glomerular
basement membrane.

APPLICATION: Use to induce experimental autoimmune glomerulonephritis (EAG) in Wistar Kyoto (WKY) rats.
QUANTITY: 1 mg
FORM: Lyophilized powder
SOURCE: Bovine kidney glomerular basement membrane
MOLECULAR WEIGHT: N/A
PURITY: N/A
USAGE: Please refer to Chondrex, Inc.’s “Inducing Experimental Autoimmune Glomerulonephritis in
WKY Rats” protocol for more details.
Inducing EAG by immunizing rats with the NC1 fraction of type IV collagen:
Animals: 7-8 weeks or older WKY rats. Sensitivity to EAG may vary depending on
the sub-strain of WKY rat, animal vendor, or facility.
Antigen: Dissolve one vial of NC1 fraction in 1 ml of PBS.
Emulsion: Make an emulsion using 1 mg/ml of NC1 fraction with an equal volume of
complete Freund’s adjuvant (Cat # 7008 - M. Tuberculosis, 1 mg/ml).
Immunization: Inject 0.2 ml (NC1 fraction - 0.1 mg) of the emulsion, subcutaneously at the
base of the rat tail.
Evaluating EAG: Measure a 16-hour proteinuria to determine the severity of EAG once a
week after immunization. Chondrex, Inc.’s Rat Urinary Protein Assay Kit
(Cat # 9040) is recommended for the proteinuria assay. Proteinuria is
usually observed on day 14 - 21 in susceptible WKY rats and reaches a
maximum level on day 21 - 28. Chondrex, Inc. recommends observing the
rats until day 35.
STORAGE: 4°C in the dark
STABILITY: 1 year
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