Concentrating Solution for Sirius Red Total Collagen Detection Kit

Concentrating Solution for Sirius Red Total Collagen Detection Kit - Cat Number: 90626 From Chondrex.

Research Field: ECM

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Sample concentration solution for Chondrex, Inc.’s Sirius Red Total Collagen Detection Kit (Cat
# 9062)
QUANTITY: 50 ml
FORM: Polyethylene glycol solution
USAGE: Collagen levels in culture media are generally lower than the standard range of the Sirius Red
Total Collagen Detection Kit (Cat # 9062). This concentrating solution (Cat # 90626) and the
following protocol can be used for concentrating culture media samples.
1. Take 1 ml culture medium.
2. Add 250 µl of Concentrating Solution.
3. Vortex and incubate at 4°C for 16-24 hours.
4. Centrifuge at 10,000 rpm for 3 minutes.
5. Discard supernatant.
6. Add 100 µl of 0.05M acetic acid to dissolve the pellet. Use this solution as your sample.
7. The calculated collagen concentration should be multiplied by a 0.1 dilution factor.
STORAGE: -20°C
STABILITY: 2 years
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REFERENCES: N/A