Dextran Sulfate Sodium for Colitis

Dextran Sulfate Sodium for Colitis - Cat Number: 4015 From Chondrex.

Research Field: Colitis

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Dextran Sulfate Sodium (DSS) for Colitis
APPLICATION: Use to induce experimental colitis in mice (1-4).
QUANTITY: 20 g
FORM: White powder
MOLECULAR WEIGHT: 40,000 (Average)
IN VIVO PROTOCOL: Feed mice DSS (3.5-5%) solution in water, ad libitum for 5-7 days.
Assessment of mice include:
1. Body weight
2. Stool consistency (wet, dry, bloody)
3. Paracellular intestinal permeability with fluorescent-labeled dextrans (Cat # 4009, 4013,
4014).
4. Hemoglobin levels, a marker of anemia due to blood loss (Cat # 6024).
5. (Optional) Transcellular intestinal permeability with D-xylose Assay Kit (Cat # 6601).
STORAGE: Room Temperature
STABILITY: 1 year
NOTES: N/A

REFERENCES:

1. R. Boismenu, Y. Chen, Insights from mouse models of colitis. J Leukoc Biol 67, 267-78 (2000).
2. M. Perše, A. Cerar, Dextran sodium sulphate colitis mouse model: traps and tricks. J Biomed Biotechnol 2012, 718617 (2012).
3. Y. Yan, V. Kolachala, G. Dalmasso, H. Nguyen, H. Laroui, et al., Temporal and spatial
analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis. PLoS One 4, e6073 (2009).
4. P. Carter, M. Watts, M. Kosloski-Davidson, K. Prasai, M. Grisham, N. Harris, et al., Iron status, anemia, and plasma erythropoietin levels in acute and chronic mouse models of colitis. Inflamm Bowel Dis 19, 1260-5 (2013).