Incomplete Freund's Adjuvant

Incomplete Freund's Adjuvant - Cat Number: 7002 From Chondrex.

Research Field: In vivo Research

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

DESCRIPTION: Mineral oil
APPLICATION: Use as an adjuvant for the immunization of antigen in experimental animals. This incomplete
Freund’s adjuvant (IFA) is suitable for collagen-induced arthritis (CIA) in rats and can also be
used for booster injections of type II collagen to increase the incidence of CIA in mice.
NOTE: Use IFA instead of complete Freund’s adjuvant (CFA) to emulsify type II collagen for
immunizing rats as rats are susceptible to adjuvant induced arthritis. To efficiently induce
arthritis in rats, muramyl dipeptide (MDP, Cat # 9065) can be added to IFA.
QUANTITY: 5 ml
FORM: Solution
EMULSION: Emulsify with an equal volume of collagen using an electric homogenizer. The syringe-syringe
method is not recommended to make collagen-IFA emulsions for inducing arthritis, but it can
be used for obtaining anti-sera.
STORAGE: 4⁰C in the dark
STABILITY: 1 year
NOTES: N/A
REFERENCES: C. William, et al. J. Exp. Med. 162: 1878-1891 (1985)
D. Trentham, et al. J. Exp. Med. 146: 857-868 (1977)
J. Taurog, et al. J. Exp. Med. 162: 962-978 (1985)
T. Koga, et al. Arth. Rheum. 23: 993-997 (1980)