ig 5-alpha Chemically Competent Cells

Description

Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.

Specifications

Competent cell type:        Chemically competent
Derivative of:                    5-alpha
Species:                            E. coli
Format:                             Tubes
Transformation efficiency:      ≥1 x 10⁹ cfu/µg pUC19
DNA
Blue/white screening:           Yes
Shipping condition:               Dry ice

Reagents Needed for One Reaction

ig™ 5-alpha chemically competent cells:  50 µl
DNA (or pUC19 Control, 10 pg/µl):           1 µl
Recovery medium:                                   1 ml

Storage

ig™ 5-alpha competent cells:   -80 ºC
pUC19 control DNA:                 -20 ºC
Recovery medium:                       4 ºC

Genotype

Φ80 Δ(lacZ)M15 fhuA2 Δ(argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17

Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol. Transformation efficiency should be greater than 1×10⁹ CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using ig™ 5-alpha chemically competent cells.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

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