Cryopreservation Medium for Cell and Tissue Viability


CryoStor® CS10 – 10% DMSO Cryopreservation Solution

CryoStor® CS10 is a pre-formulated, GMP-grade cryopreservation medium containing 10% DMSO designed to protect cells and tissues during freezing, storage, and thawing. It ensures high post-thaw viability and stability without the need for serum or animal-derived components, supporting consistent results in cell therapy, stem cell research, and biobanking applications.

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CryoStor® CS10: Advanced Cryopreservation Medium for Cell and Tissue Viability

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CryoStor® CS10 is a 10% DMSO-based cryopreservation solution developed to maintain cell integrity during freezing, storage, and thawing. It is widely used in cell therapy, biobanking, and research cryobiology due to its consistent performance and GMP-grade formulation.
The medium minimizes osmotic shock, reduces intracellular ice formation, and helps retain cell membrane stability during cooling and warming processes.

Composition and Design Principles

The core component, dimethyl sulfoxide (DMSO), acts as a penetrating cryoprotectant that prevents intracellular ice crystals. CryoStor® CS10 contains:

  • 10% DMSO
  • Electrolyte-balanced base medium
  • Osmotic buffering agents
  • pH stabilizers compatible with mammalian cell culture

The formula eliminates serum, animal proteins, and undefined supplements, ensuring a chemically defined and xeno-free medium, ideal for NIH human stem cell research and NIST cellular measurement programs.

Mechanism of Action

During freezing, ice nucleation and solute concentration gradients can cause severe stress to cells. Controlled-rate cooling and cryoprotectants are therefore essential. CryoStor® CS10 acts by:

  1. Reducing ice crystal formation, DMSO infiltrates the cytoplasm to minimize damage.
  2. Maintaining osmotic balance, buffers prevent dehydration and swelling.
  3. Preserving membrane potential, ionic balance supports mitochondrial and cytoplasmic integrity.
  4. Supporting rapid post-thaw recovery, as verified by PubMed Cryobiology Studies.

Application Fields

CryoStor® CS10 is used by academic laboratories, biotech companies, and clinical research centers for freezing:

  • Primary cells and stem cells.
  • Lymphocytes and CAR-T lines.
  • Induced pluripotent stem cells (iPSCs).
  • Adherent and suspension cultures.
  • Engineered cell therapy products.

Protocol Overview

  1. Pellet cells by centrifugation and remove the supernatant.
  2. Resuspend in cold CryoStor® CS10 (2–8 °C) to equilibrate osmotically.
  3. Cool at approximately, 1 °C per minute to the target temperature.
  4. Transfer to liquid nitrogen vapor phase for long-term storage.
  5. Thaw rapidly at 37 °C and dilute to remove DMSO within 5 minutes.

Compatibility and Validation

CryoStor® CS10 has been validated for :

  • Flow cytometry recovery assays.
  • Stem cell pluripotency retention.
  • Membrane integrity tests.
  • Post-thaw viability studies using Trypan Blue and ATP assays.

Advantages Over Conventional Cryopreservation Media

cryostor

Parameter
 CryoStor® CS10
 Conventional Media
Composition
 Defined, serum-free
 Variable, serum-based

DMSO Concentration

 10%
 5–15%, inconsistent
GMP Certification
 Yes
 Often absent
Cell Viability Post-Thaw
 >85%
 ~60–70%
Regulatory Traceability
 Full documentation
 Often limited

Conclusion

CryoStor® CS10 is engineered to protect biological materials through every phase of cryopreservation. Its chemically defined formulation, regulatory consistency, and proven post-thaw viability make it an essential tool for modern cell and gene therapy workflows.

For researchers in regenerative medicine, immunotherapy, and advanced bioprocessing, CryoStor® CS10 provides reliability backed by science and compliance.

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