Avantor Protein A Mix-N-Go ELISA Kit | F965

Avantor Protein A Mix-N-Go ELISA Kit is intended for use in quantitating the recombinant Protein A ligands used in the Avantor J.T.Baker® BAKERBOND® PROchievA™ chromatographic resins. It is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mix-N-Go™ assay for Avantor J.T.Baker® BAKERBOND® PROchievA™ Protein A is a two-site immunoenzymetric assay. Samples containing Avantor J.T.Baker® BAKERBOND® PROchievA™ Protein A ligands are first diluted in the Mix–N-Go Sample Diluent provided with the kit. The Mix-N-Go Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. 800-F965 Mix-N-Go for Avantor J.T.Baker® BAKERBOND® PROchievA™ Protein A ELISA Product Insert 2 CYG-LBL-00424 Rev: 03 Eff: 09 Dec 2020 The samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A:HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present in the sample. Accurate quantitation is achieved by comparing the signal of unknown samples and controls to Avantor J.T.Baker® BAKERBOND® PROchievA™ Protein A Standards assayed at the same time.