CAP® cell HCP ELISA Kit | F820
- SKU:
- 214-F820
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Target Expression System:
- CAP® Cell
- Species Group:
- Mammalian
- Species:
- Human
- Assay format:
- 96 Well Plate
- Time to result:
- ~2 hrs. 50 min
- LOD:
- ~0.9 ng/mL
- LLOQ:
- ~1.5 ng/mL
- Recommended Diluent:
- I094
Description
CAP® cell HCP ELISA Kit | F820
CAP® cell HCP ELISA Kit is intended for use in determining the presence of host cell protein impurities in products manufactured by expression in the CAP® human cell line. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The CAP® cell line assay is a two-site immunoenzymetric assay. Samples containing CAP® cell HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-CAP® cell antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture anti-CAP® cell antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of CAP® cell line HCPs present.
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