Human IgM ELISA Kit | F170
- SKU:
- 214-F170
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Assay format:
- 96 Well Plate
- Time to result:
- ~2 hrs. 50 min
- LOD:
- ~177 pg/mL
- LLOQ:
- 250 pg/mL
- Recommended Diluent:
- I028
Description
Human IgM ELISA Kit | F170
Human IgM ELISA Kit is intended for use in quantifying very low concentrations of human immunoglobulin M (hIgM). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The hIgM assay is a two-site immunoenzymetric assay. Samples containing hIgM are reacted in microtiter strips coated with an affinity purified capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled anti-hIgM antibody, is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-hIgM-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of hIgM present.
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