JSR Protein A ELISA Kit | F740

JSR Protein A ELISA Kit is intended for use in quantitating the recombinant protein A ligands used in JSR Life Sciences’ AmsphereTM protein A chromatography resin series. It is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mix-N-Go assay for AmsphereTM Protein A is a twosite immunoenzymetric assay. Samples containing AmsphereTM Protein A ligands are first diluted in the Mix–N-Go Sample Diluent provided with the kit. The Mix-NGo Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A: HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present in the sample. Accurate quantitation is achieved by comparing the signal of unknowns to AmsphereTM Protein A Standards assayed at the same time.