Mouse IgG2a ELISA Kit | F046

Mouse IgG2a ELISA Kit is intended for use in quantitating mouse IgG2a. The antibodies used in this kit are specific for mouse IgG2a and will not cross react significantly with most immunoglobulins from other species or with other mouse immunoglobulins. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mouse IgG2a assay is a two-site immunoenzymetric assay. Samples containing mouse IgG2a are reacted in microtiter strips coated with an affinity purified anti-IgG2a capture antibody. A second horseradish peroxidase enzyme labeled goat anti-mouse IgG2a antibody is reacted forming a sandwich complex of solid phase antibody-mouse immunoglobulin enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of mouse IgG2a present. Accurate quantitation is achieved by comparing the signal of unknowns to IgG2a standards assayed at the same time.