Mouse IgM ELISA Kit | F090

Mouse IgM ELISA Kit is intended for use in quantitating mouse IgM. The antibodies used in this kit are specific for Mouse IgM and will not cross react significantly with most immunoglobulins from other species or with other mouse immunoglobulins. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mouse IgM assay is a two-site immunoenzymetric assay. Samples containing mouse IgM are reacted in microtiter strips coated with an affinity purified anti-IgM capture antibody. A second horseradish peroxidase (HRP) enzyme labeled goat anti-mouse IgM antibody is reacted forming a sandwich complex of solid phase antibody- mouse immunoglobulin-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of mouse IgM present. Accurate quantitation is achieved by comparing the signal of unknowns to IgM standards assayed at the same time.